ScriptGuard™ RNase Inhibitor

Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C

ScriptGuard™ RNase Inhibitor prevents the degradation of RNA by eukaryotic RNase A, RNase B andRNase C, but it does not inhibit RNase 1, RNase H, RNase T1, RNase T2 or S1 Nuclease. A potentaffinity for RNases (Ki ~10-14 M) and a 1:1 binding ratio ensures rapid inhibition even when trace amountsof RNase are present. ScriptGuard RNase Inhibitor is free of detectable RNase and DNase activities andmammalian DNA. Because it does not interfere with most commonly used enzymes. It can be used in awide variety of applications, including cDNA synthesis, and in vitro transcription and translation reactions.

ScriptGuard RNase Inhibitor is supplied in a 50% glycerol solution containing 50 mM Tris-HCl,pH 7.5, 100 mM NaCl, 10 mM DTT, 0.1 mM EDTA and 0.1% Triton® X-100.

Unit Definition One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Functional Testing ScriptGuard RNase Inhibitor is functionally tested to inhibit hydrolysis of cyclic 2',3'-CMP by RNase A. Contaminating Activity Assays ScriptGuard RNase Inhibitor is free of detectable mammalian DNA, and RNase and DNase activities.