乙醇脱氢酶 来源于酿酒酵母

醇脱氢酶

ADH | Alcohol Dehydrogenase from yeast | Alcohol:NAD+ oxidoreductase | alcohol dehydrogenase | aldehyde reductase | Alcohol dehydrogenase | ADH1 | ADC1

A124756-5KU

5KU

EnzymoPure™

≥300 units/mg protein

醇脱氢酶与乳酸脱氢酶已一起用于使用钠(R,S)-[2-3H]乳酸盐酶促还原乙醛的过程中。在预去除醛基后使用醇脱氢酶对NAD的酶促还原进行检测，以对乙醇浓度进行比色测定。 Protein Accession Number: P00330 CATH Classification (v. 3.2.0): Class: Alpha Beta Architecture: Alpha-Beta Complex and 3-Layer (aba) Sandwich Topology: Quinone Oxidoreductase; Chain A, domain 1 and Rossmann fold Molecular Weight: 146.8 kDa (Theoretical) Optimal pH: 5.4 (Shore and Theorell 1966) Isoelectric Point: 6.23 (Theoretical) Extinction Coefficient: 189,320 cm-1 M-1 (Theoretical) E1%,280 = 12.89 (Theoretical) Activators: Sulfhydryl activating reagents Mercaptoethanol Dithiothreitol Cysteine Heavy metal chelating agents (White and White 1997) Inhibitors: Heavy metals and -SH reagents Thiourea Purine and pyrimidine derivatives Chloroethanol and fluoroethanol N-alkylmaleimides Iodoacetamide 1,10-phenanthroline 8-hydroxyquinoline Beta-NAD analogs (White and White 1997) Applications: Enzymatic determination of primary alcohols, and aldehydes Synthesis of chiral compounds Spectrophotometric assay of plasmalogenase Enzymatic catalysis in organic solvents Studies of NAD+, NADH, NADP+, and NADPH

≥300 units/mg protein

天然

发面酵母(Baker's yeast)