8-Azanebularine, a compound with hydrogen in place of the C6 amino group, inhibits the ADAR2 reaction at high concentrations ( IC 50 =15 mM). 8-Azanebularine is incorporated into an RNA structure recognized by human ADAR2 results in high-affinity binding ( K D =2 nM). 8-Azanebularine can be used for the research of ADAR-catalyzed RNA-editing reaction Form:Solid IC50& Target:ADAR2 reaction 15 mM (IC 50 ) ADAR2 2 nM (Kd)