Asukamycin, a manumycin-type metabolite, could be isolated from Streptomyces nodosus subsp. asukaensis. Asukamycin is an antibiotic and has antimicrobial activity. Asukamycin inhibits growth of various tumor cell lines. In Vitro Asukamycin (1-50 μM; 24 h) in a concentration-dependent manner reduces viability of five different human cell lines with IC 50 of 1-5 μM. Asukamycin (50 μM; 24 and 72 h; THP-1 cells) causes a significant increase in intracellular caspase 8 and caspase 3 activity. Asukamycin (0-100 mcg/mL; bacteria, 18 h; fungus, 72 h) has antimicrobial activity. Asukamycin inhibits the growth of Gram-positive bacteria within a concentration range of 0.78-12.5 mcg/mL and inhibits the growth of Trichophyton mentagrophytes at a concentration of 25 mcg/ mL. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Human myeloid THP-1, HL-60, U-118 MG, U-87 MG and U-937 cells Concentration: 1-50 μM Incubation Time: 24 hours Result: Reduced viability of five different cell lines in a concentration-dependent manner. In Vivo Asukamycin (0-450 mg/kg; i.p.; mice) has low toxicity in vivo. Asukamycin has acute toxicity with an LD 50 value of 48.5 mg/kg by intraperitoneal injection. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: MiceDosage: 0-450 mg/kg Administration: Intraperitoneal injection Result: Had no effect on mice when administered by 450 mg/kg and the acute toxicity (LD 50 ) was 48.5 mg/kg by intraperitoneal injection. Form:Solid