Recombinant IRF3 Antibody

抗体

干扰素调节因子3抗体 | 干扰素调节因子3单克隆抗体 | 干扰素调节因子3重组单克隆抗体 | IRF3抗体 | IRF3单克隆抗体 | IRF3重组单克隆抗体

IIAE7 antibody | Interferon regulatory factor 3 antibody | IRF 3 antibody | IRF-3 antibody | IRF3 antibody | IRF3_HUMAN antibody | MGC94729 antibody | IRF3 | IIAE7

Liquid

Ab111210-10μl

10μl

重组

见COA

-20°C储存,避免反复冻融

超低温冰袋运输

短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。

10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4

0.02% Sodium azide

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. Post-translational: Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

A synthetic peptide derived from human IRF3 (AA 82-123).

兔(Rabbit)

人(Human), 大鼠(Rat), 小鼠(Mouse) | 人(Human),大鼠(Rat),小鼠(Mouse)

IRF3

WB: HeLa, Jurkat, Hep G2 cell lysates. IHC: Human hodgkin's lymphoma, astrocytoma, ovarian cancer, cervix carcinoma tissue. IF/ICC: HeLa, Jurkat cells.

150 kDa

Unconjugated

Rabbit IgG

重组抗体

kappa

Protein A purified

此产品可替替代Ab111215

Whole IgG