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名称
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody
分子类型
抗体
别名
Flag标签抗体 | DYKDDDDK表位标签 | DYKDDDDK抗体
英文别名
DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C
货号
Ab220320-10μg
包装规格
10μg
级别
无动物源
浓度
≥95%(SDS-PAGE)
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融.
储存缓冲液
50% Glycerol, 150 mM NaCl, 10 mM sodium phosphate, pH 7.4
生化机理
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended.
免疫原
Synthetic DYKDDDDK peptide
宿主种属
小鼠(Mouse)
种属反应性
不依赖种属(Species independent)
特异性
DYKDDDDK epitope tag
阳性对照
WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag tag IF/ICC: HEK293 cells transfected with Flag and mCherry tags, HEK293 cells transfected with Flag and eGFP tags IP: HEK293 cells transfected with F
SDS-PAGE
150.0 KDa
偶联
Unconjugated
亚型
Mouse IgG1
克隆类型
重组抗体
轻链亚型
kappa
纯化方法
Protein A purified
克隆号
P16A17
Format
Whole IgG
危险属性
「暂无危险属性」
上下游信息
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技术文档
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相关文章
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Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody 无动物源 10μg

品牌:阿拉丁
货号:Ab220320-10μg
级别: 无动物源
货期:30天
已售 12 件
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10μg

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