(Aminomethyl)phosphonic acid is a metabolite of amitrole and glyphosate herbicides and was analyzed by HPLC using an electrochemical detector.(Aminomethyl)phosphonic acid was used to develop a novel kind of immobilized metal affinity chromatography column based on organic-inorganic hybrid silica monolith. (Aminomethyl)phosphonic acid is a metabolite of amitrole and glyphosate herbicides and was analyzed by HPLC using an electrochemical detector. (Aminomethyl)phosphonic acid was used to develop a novel kind of immobilized metal affinity chromatography column based on organic-inorganic hybrid silica monolith.