BRD7586 is a cell-permeable small-molecule inhibitor of SpCas9. BRD7586 is the smallest known anti-CRISPR. In Vitro BRD7586 specifically engages SpCas9 but not Cas12a in cells. BRD7586 (0-20 μM; 24 h) dose-dependently inhibits SpCas9 in the HiBiT-knock-in assay in HEK293T cells. BRD7586 (20 μM; 48 h) enhances the ratio of on-target versus off-target in HEK293T cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid