Biotin-16-UTP (10mM)

生物素-16-UTP | 生物素-16-鸟苷三磷酸

Biotin-16-UTP | Biotin-16-(5-aminoallyl)-UTP；Biotin-16-Aminoallyluridine-5'-Triphosphate | Biotinyl-ε-aminocaproyl-γ-aminobutyryl-5-(3-aminoallyl)-uridine-5'-triphosphate

Biotin-16-UTP (10mM)

B744152-20μl

20μl

BioReagent

≥95%

-20°C储存,避免反复冻融

超低温冰袋运输

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

储存缓冲液：Ultrapure water 是否无菌：否 产品介绍： Biotin-16-UTP在大多数情况下可以取代UTP作为各种RNA合成酶、修饰酶、聚合酶，包括T3、T7或SP6 RNA聚合酶等的底物，对RNA实现Biotin标记。生物素标记的RNA探针可在许多应用中取代放射性标记RNA探针，包括原位杂交和染色体定位等。生物素化探针可以使用基于链霉亲和素结合的多种方法进行检测，如可以使用荧光标记的链霉亲和素直接检测标记RNA；也可以使用链霉亲和素偶联HRP或碱性磷酸酶或链霉亲和素偶联抗体间接检测标记的RNA。 Storage buffer solution: Ultrapure water Is it sterile: No Product Introduction: Biotin-16-UTP can replace UTP in most cases as a substrate for various RNA synthases, modifying enzymes, polymerases, including T3, T7, or SP6 RNA polymerases, to achieve Biotin labeling of RNA. Biotin labeled RNA probes can replace radiolabeled RNA probes in many applications, including in situ hybridization and chromosome localization. The biotinylated probe can be detected using various methods based on streptavidin binding, such as directly detecting labeled RNA using fluorescently labeled streptavidin; Alternatively, labeled RNA can be indirectly detected using streptavidin conjugated HRP, alkaline phosphatase, or streptavidin conjugated antibodies.

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