BCIP^®/NBT-蓝色液体膜底物系统 | BCIP ® /NBT-蓝色液体膜底物系统

B755440-100ml

100ml

alkaline phosphatase substrate

BCIP®/NBT蓝液底物系统已用于碱性磷酸酶(ALP)活性的比色检测，如蛋白质印迹分析。应用： 硝基蓝四唑（NBT）与碱性磷酸酶底物5溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于免疫印迹和免疫组织学染色程序。这些底物系统能够产生不溶的NBT二甲双胍终产物，呈蓝色至紫色并且可以在视觉上观察到。BCIP®/NBT蓝液底物系统是一种专门配制的稳定液体酶底物，针对印迹应用进行了优化，方便省时，一致性高。 Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency. Application: BCIP/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.