BCIP^®/NBT液体底物系统 | BCIP ® /NBT液体底物系统

B755514-100ml

100ml

ready to use solution

BCIP®/NBT液体底物系统用于：对骨髓基质细胞中的碱性磷酸酶（ALP）进行染色用作Western 印迹检测的底物，检测基质金属蛋白酶-2特异性抗体作为酶联免疫斑点（ELISpot）测定中的底物，测定链霉抗生物素蛋白碱性磷酸酶碱性磷酸酶的即用型沉淀底物系统。该底物系统能够产生不溶的NBT二甲双胍终产物，其颜色为蓝色至紫色并且可以在视觉上观察到。使用BCIP/NBT的免疫组织化学染色需要有机封固剂，并且可以用核固红或亮绿复染。BCIP/NBT通常比固红更灵敏。 不建议用于ELISA（微孔）检测。 A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures. BCIP/NBT Liquid Substrate System has been used: for staining alkaline phosphatase(ALP) in bone marrow stromal cells as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays