胶原酶 NB 4G 来源于溶组织梭菌

Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene

C279008-100mg

100mg

EnzymoPure™

证明等级,≥ 0.18 U/mg(PZ activity* acc. to Wünsch, 25 °C)

描述 胶原酶NB 4G是一种粗制胶原酶，它包含胶原蛋白水解和其他酶促活性，包括梭菌蛋白酶和中性蛋白酶。这些活动的均衡比例导致温和有效的组织解离。适用于分离多种细胞类型，尤其是成纤维细胞，人和啮齿动物肝细胞，蛙卵母细胞和上皮细胞。 应用 胶原酶NB 4G适用于多种组织类型的解离。 规格 包含I类和II类胶原酶（1）以及中性蛋白酶，梭菌蛋白酶和类胰蛋白酶活性的平衡混合物。 活性：≥0.18 U / mg （Clostridiopeptidase A） EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenase) • CAS [9001-12-1] 参考和定义 单位定义： 1 U在25°C，pH 7.1下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰基-L-亮氨酰甘氨酰-L-脯氨酰基-D-精氨酸的水解。 References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51 Collagenase NB 4G is a crude collagenase which contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities leads to gentle and efficient tissue dissociation.Suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells. Application Collagenase NB 4G is suitable for dissociation of a broad variety of tissue types. Specifications Contains class I and class II collagenase (1) and a balanced mix of neutral protease, clostripain and trypsin-like activities. Activity (Wünsch): ≥ 0.18 U/mg (Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenase) • CAS [9001-12-1] References and Definitions Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51