MFI8 is a small molecule inhibitor of mitochondrial In Vitro MFI8 (20 μM; 6 h) reduces significantly mitochondrial aspect ratio with an EC 50 of 4.8 μM, and inhibits mitochondrial function via mitofusins and subsequently promoting mitochondrial fission. MFI8 possesses functional groups that meet the criteria of the pharmacophore model to interact with mitofusins and promotes mitochondrial fission. MFI8 binds to the HR2 domain of MFN2, modulates MFN conformation and complexes, and alters mitochondrial functionality. MFI8 (0-20 μM; 6 h) concentration-responsively increases caspase-3/7 activity, induces cytochrome c release and decreases membrane potential in a mitofusin-dependent manner. MFI8 also induces DNA damage and induces cell death in combination with BV6 SMAC (second mitochondria-derived activator of caspases) mimetic. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid IC50& Target:Mitofusin