Cas9 NLS (SpCas9-NLS)

Cas9 NLS（SpCas9-NLS）

CRISPR-associated endonuclease Cas9 | Csn1 | cas9

C744421-500pmol

500pmol

无动物源

20μM (~3.2μg/μL)

阿拉丁生产的 Cas9 NLS (SpCas9-NLS)，即含有核定位信号的 CRISPR-associated endonuclease Cas9(也称 Csn1)，是本公司自主研发的技术平 台表达、纯化获得的一种来源于 Streptococcus pyogenes，能在 gRNA 引导下序列特异性地切割双链 DNA 的核酸内切酶。本产品可以用于细胞 内的 CRISPR/Cas9 系统介导的基因编辑，也可以用于体外筛选高效的 guide RNA (gRNA)序列、特定 DNA 序列在 gRNA 引导下的剪切、含有特定 序列的双链环形 DNA 的线性化等用途。Cas9 NLS 在 Cas9 蛋白的 N 端和 C 端都含有 SV40 T 抗原的核定位序列(Nuclear localization signal or nuclear localization sequence, NLS)，使 Cas9 与 gRNA 形成的复合物在转染进入细胞后、能迅速地从细胞质进入到细胞核内，从而大大地提高 了基因编辑的效率。Cas9 NLS 可以通过显微注射、电穿孔和脂质体介导等方法进入细胞，而这种不需要使用 DNA 的系统不会产生外源 DNA 整合 至细胞基因组的风险 。CRISPR/Cas9 是一项突破性的基因组编辑技术，操作便捷，应用广泛。CRISPR/Cas9 系统由 Cas9 Nuclease 和 gRNA 复 合物所组成。gRNA，也称 sgRNA (single guide RNA)，由 18-20bp 与靶基因序列互补的 CRISPR RNA (crRNA)序列以及能与 Cas9 特异性结合的 trans-activating crRNA (tracrRNA)序列组成。 纯度（Purity）： 不含 DNA 外切酶，不含非 gRNA 依赖的 DNA 内切酶，不含 RNA 酶。 组分和说明 C744421

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids)CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity .Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference).

重组表达

E. coli Accession #: Q99ZW2.1 | E. coli

化脓性链球菌血清型M1(Streptococcus pyogenes serotype M1)

Yes

Yes