细胞色素 C 来源于马心脏

C755620-10mg

10mg

UltraBio™

≥99%(SDS-PAGE)

来自马心脏的细胞色素c已被用于制备合成和标定生物素化细胞色素 c的储备溶液。 它还被用作反应缓冲液的成分，该缓冲液可用于染色电子传输链（ETC）复合物IV及用蓝色非变性凝胶电泳确定该复合物活性。马的细胞色素c经过H2O2和UV 处理后的特异性位点和氧化程度已经过表征。研究表明这些位点可能可用作细胞内氧化环境的生物标记。应用： 细胞色素C(Cyt C)是一种核DNA编码蛋白。它来源于它的前体脱血红素细胞色素 c，并可以穿过线粒体外膜。研究领域：细胞凋亡 Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria. Research area: Apoptosis Application: Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis. The specific sites and extent of oxidation in horse cytochrome c treated with H 2 O 2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

细胞色素 C（Cyt C）通过激活半胱天冬酶 3（一种死亡蛋白酶），充当细胞凋亡的有效信号分子。它在血红素结合中发挥作用，有细胞色素 c血红素裂解酶的帮助加速了细胞色素 c释放到线粒体膜间隙中。 Cyt C还通过与心磷脂相互作用表现出脂质结合的能力，心磷脂是一种磷脂，可启动蛋白质展开并激活Cyt C中的过氧化物酶功能。细胞色素c的主要身份是电子载体线粒体蛋白。在细胞内，细胞色素c能够在二价铁和三价铁之间转化，使得它称为了有效的生物电子载体，在动植物细胞内氧化中起到很重要的作用。它通常被视作呼吸作用催化剂，在呼吸底物和氧气之间形成必需的电子桥