胱硫醚-β-裂解酶 (CBL)

胱硫醚裂解酶 | METC

Cystathionine beta-lyase | Cystathionine betalyase | Cystathionine β-lyase | METC

C774055-1KU

1KU

生物活性

≥95%(SDS-PAGE)

Cystathionine-β-lyase (CBL, EC 4.4.1.8) represents a pivotal member of the lyase family, demonstrating exceptional substrate specificity. The enzyme catalyzes the β-cleavage of L-cystathionine with high precision, generating homocysteine (Hcy) through the sequential elimination of pyruvate and ammonia moieties. Reaction Synergistic Catalytic System with CBS‌ 1.CBL forms an efficient catalytic tandem with cystathionine-β-synthase (CBS, EC 4.2.1.22, Aladdin No. C774059) in the cyclic Hcy detection mechanism: ‌2. Condensation Phase ‌: CBS initiates the reaction by condensing Hcy with serine to form cystathionine. 3.Cleavage Phase ‌: CBL then cleaves the cystathionine molecule to regenerate Hcy. This cyclic amplification strategy enables highly sensitive quantitative Hcy determination. Consequently, CBL serves as the cornerstone enzyme in cystathionine-based Hcy detection kit development while also finding utility in other enzymatic applications requiring β-specific cleavage catalysis. Properties Isoelectric point: 6.0 Michaelis constant: 2.8×10^-4mol/L (L-cystathionine) Optimum pH: 8.0 Fig. 1 Optimum temperature: 40℃ Fig. 2 pH Stability: 6.0~9.0 (25℃, 24hr) Fig. 3 Thermal stability: Inhibitors: Cu2+ Effect of various chemicals: Table 1 Reaction：

≥90 U/mg protein; Unit definition: One unit of activity is defined as the production of 1μmol of L-homocysteine per min at 37℃ at pH8.0.

重组表达

45 kDa (SDS-PAGE)

E. coli Accession #: F5A553 | E. coli

微生物(Microorganism)