DNA酶I

脱氧核糖核酸酶 I

D128586-10KU

10KU

重组

≥5000 units/mg protein；来源于牛胰腺

用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drμgs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

DNase I 是一种内切酶，可作用于嘧啶附近的磷酸二酯键，生成末端带有 5′-磷酸的多核苷酸。在 Mg2+ 存在的情况下，DNAse I 可独立裂解 DNA 的每一条链，裂解位点是随机的。在 Mn2+ 存在的情况下，两条 DNA 链会在大致相同的部位被裂解。Mn2+、Ca2+、Co2+ 和 Zn2+ 等二价阳离子是该酶的活化剂。浓度为 5 mM 的 Ca2+ 能使酶稳定，防止蛋白水解。最佳 pH 值为 7 至 8。牛胰腺中的 DNAse I 由 A、B、C 和 D 四种色谱法可区分的成分组成，它们的摩尔比分别为 4:1:1。其中只有少量的 D。已知 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）6 和肌动蛋白7 可抑制酶的活性。

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