DBF被细胞色素P450（特别是CYP2C8，CYP2C9，CYP2C19和CYP3A）脱烷基形成荧光素酯。加入碱后可以定量荧光荧光素产物，该碱会水解酯并增加pH值，以实现最佳的荧光素荧光检测。 DBF is dealkylated by cytochromes P450 (especially CYP2C8, CYP2C9, CYP2C19, and CYP3A) to form a fluorescein ester. The fluorescent fluorescein product can be quantitated after the addition of base, which hydrolyzes the ester and increases the pH for optimal fluorescein fluorescence detection.