脱氧核糖核酸酶 I 来源于牛胰腺

D755012-10KU

10KU

Type II, lyophilized powder, Protein≥80 %,≥2,000 units/mg protein

DNAse I可用于切开DNA，作为将标记碱基插入DNA的第一步。来自Sigma的酶已在Madin-Darby犬肾（MDCK）II细胞系的细胞裂解物制备中与RNAse一起用于核酸酶的储备。它也用于从辛德比斯病毒中提取RNA。来自牛胰腺的脱氧核糖核酸酶I已用于两种类似于脱氧核糖核酸酶I的新型人类基因的鉴定、定位和表达。来自牛胰腺的脱氧核糖核酸酶I也已用于研究可从牛胰腺中获得高活性RNase、DNase、胆固醇酯酶和胰蛋白酶的新方法。用于从蛋白质样品中除去 DNA。 DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. Used for the removal of DNA from protein samples.

DNase I是一种内切核酸酶，可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg 2+ 存在时，DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn 2+ 存在时，两条DNA链都几乎是在同样的位置被切割。 最适pH在7-8之间。二价阳离子如Mn 2+ , Ca 2+ , Co 2+ 以及Zn 2+ 都是酶的激活剂。 5 mM浓度的Ca 2+ 可稳定酶免收蛋白水解酶的消化。来自牛胰腺的DNAse I由四种色谱可区分的组分A，B，C和D组成，它们的摩尔比分别为4:1:1。仅有少量的D被发现。 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS） 和肌动蛋白 都已知可抑制酶的活性。

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