脱氧核糖核酸酶 I 来源于牛胰腺

D755013-1vial

1vial

Type II-S, lyophilized powder, Protein≥80 %,≥2,000 units/mg protein

Sigma公司的DNAse I与人类尿液来源的白细胞介素1抑制剂相比，具有水解[14C]DNA [14C]的能力。它也被用来切割139个碱基对Hind III/Nci I限制性片段，以研究该酶用于足迹实验的稳定性。DNase I被广泛用作研究药物和蛋白质与DNA结合的印迹剂。已有研究采用牛胰腺脱氧核糖核酸酶I，研究了从牛胰腺中提取高活性RNase、DNase、胆固醇酯酶和胰蛋白酶的一个新途径。牛胰腺脱氧核糖核酸酶I也被用于研究鲤鱼肝脏DNase的分离和进一步的特性。用于从蛋白质样品中除去 DNA。 DNAse I from Sigma has been compared with human urine-derived interleukin 1 inhibitor for the ability to hydrolyze [ 14 C]DNA [ 14 C]DNA. It has also been used to cleave a 139 base pair Hind III/Nci I restriction fragment to investigate the stability of the enzyme for use in footprinting experiments. DNase I is widely used as a footprinting agent for studying drug and protein binding to DNA. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the isolation and further characterization of carp liver DNase. Used for the removal of DNA from protein samples.

DNase I是一种核酸内切酶，作用于嘧啶附近的磷酸二酯键，产生末端为5-磷酸的多核苷酸。在Mg 2+ 存在时，DNAse I对每条DNA链进行独立的裂解，裂解位点是随机的。在Mn 2+ 存在下，两条DNA链几乎在同一位点被裂解。 其最佳pH值在7到8之间。二价阳离子，如Mn 2+ 、Ca2+ 、Co 2+ 和Zn 2+ 是该酶的激活剂。 浓度为5mM的Ca 2+ 可以稳定酶的蛋白水解消化。牛胰腺DNA酶I由A、B、C、D四种色谱可分辨组分组成，摩尔比分别为4:1:1。仅发现少量的D。 2-巯基乙醇、螯合剂、十二烷基硫酸钠(SDS) 和肌动蛋白 是已知的抑制该酶活性的物质。

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