脱氧核糖核酸酶 I 来源于牛胰腺

D755016-10mg

10mg

lyophilized powder, Protein≥85 %,≥400 Kunitz units/mg protein

从原代细胞分离液中去除DNA，在降低粘度的同时提高产量：将标记碱基掺入DNA：DNA缺口放射性标记生物工艺应用：DNA去除在RT-PCR前从RNA制剂中去除基因组DNA体外转录缺口平移DNase足迹肌动蛋白调控胞内肌动蛋白聚合和细胞凋亡蛋白质与核酸紫外线交联DNase参与调控胞内肌动蛋白聚合，同时参与细胞凋亡过程 [1]用于从蛋白质样品中除去 DNA。应用： DNase I（脱氧核糖核酸酶I）是一种从牛胰腺分离的核酸内切酶。 我们的DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。牛胰腺DNase存在四种异构酶A, B, C和D，均具有不同的等电点：5.22, 4.96, 5.06及4.78.3。主要形式为A，其次为B和C，D所占的比例最小。 DNase I结构类似于核酸外切酶III。其中包括两个中央β折叠。每个β折叠由六个β-链组成。这种复杂的β折叠被大量环形和螺旋形区域包围。该酶结构类似于核酸外切酶III。[1] DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas. Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides. Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D. DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. [1] Application: Decreases viscosity providing better yields by removing DNA in primary cell isolation: Incorporating labelled bases into DNA: DNA nick Radioactive labelling Bioprocessing applications: DNA removal Eliminating genomic DNA from RNA preparations before RT-PCR In vitro transcription Nick translation DNase footprinting Actin regulation of actin polymerization in cells, and cell apoptosis UV crosslinking of proteins to nucleic acids DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process [1] Used for the removal of DNA from protein samples.

DNase I是一种内切核酸酶，可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg 2+ 存在时，DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn 2+ 存在时，两条DNA链都几乎是在同样的位置被切割。 二价阳离子如Mn 2+ , Ca 2+ , Co 2+ 以及Zn 2+ 都是酶的激活剂。5 mM浓度的Ca 2+ 可稳定酶免收蛋白水解酶的消化。最适pH在7-8之间。 来自牛胰腺的DNAse I由四种色谱可区分的组分A，B，C和D组成，它们的摩尔比分别为4:1:1。仅发现少量D。 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS） 和肌动蛋白 都已知可抑制酶的活性。

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