EN40 is a potent, selective aldehyde dehydrogenase 3A1 (ALDH3A1) inhibitor as a covalent ligand, exhibits an IC 50 value of 2 uM In Vitro EN40 (10-1000 μM; 48 hours) shows inhibitory effecton ALDH3A1 activity and impairs A549 cell survival. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 cells Concentration: 10 μM; 100 μM; 1000 μM Incubation Time: 48 hours Result: Inhibited A549 cells survival. In Vivo EN40 (intraperitoneal injection; 50mg/kg; from 14 days; once per day) exerts strong anti-tumorigenic effects in established A549 tumor xenografts, shows good tolerability with no body weight loss in mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: SCID mice with A549 cells Dosage: 50 mg/kg Administration: Intraperitoneal injection; 50mg/kg; from 14 days; once per day Result: Had strong anti-tumorigenic effects in tumor xenografts. Form:Oil IC50& Target:IC50: 2 uM (ALDH3A1)