EMSA探针－AP2

EMSA Probe AP2

E745598-30μl

30μl

10μM

-20°C储存

超低温冰袋运输

-20℃保存，一年有效。

EMSA探针－AP2是用于EMSA(也称gel shift)研究的AP2 consensus oligonucleotide。这个双链寡核苷酸含有公认的AP2结合位点，可以用作EMSA研究时的探针。 AP2 consensus oligo的序列如下：, "5'-GAT CGA ACT GAC CGC CCG CGG CCC GT-3'", "3'-CTA GCT TGA CTG GCG GGC GCC GGG CA-5'", 本EMSA探针可以使用T4 Polynucleotide Kinase在γ-32P]ATP存在的情况下进行标记，也可以用于生物素标记。 一个包装的探针可以进行约90个同位素标记探针的反应，每次标记的探针可以测定约100个样品；或约可以进行60个生物素标记探针反应。如果作为未标记的探针用于同位素标记探针的冷竞争(cold competition)，可以进行90-180个冷竞争反应。如果用于生物素标记探针的冷竞争时，可以进行约30个冷竞争反应。 注意事项 ： 避免加热到40℃以上，温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1. 用于EMSA探针的标记，可以参考如下反应体系：待标记探针(1.75μM)2μlT4 Polynucleotide Kinase 10X Buffer1μlNuclease-Free Water5μl[γ-32P]ATP(3,000Ci/mmol at 10mCi/ml)1μlT4 Polynucleotide Kinase (5-10u/μl)1μl总体积10μl2.如果用于生物素标记，具体方法和步骤可以参考阿拉丁的EMSA探针生物素标记试剂盒。 3. 如果用于冷竞争，没有标记的探针的用量应该是标记探针的用量的50-100倍。 Aladdin's EMSA Probe – AP2 is an AP2 consensus oligonucleotide designed for EMSA . This double-stranded oligonucleotide contains the well-recognized binding sequence for AP2 transcription factors, and can be used as a probe for EMSA. The nucleotide sequence of AP2 consensus oligo is as follows Precautions ： Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： 1.Set up the probe labeling reaction with isotopes by adding reagents in sequence as follows:Probe to be labeled (1.75µM)2µlT4 Polynucleotide Kinase 10X Buffer1µlNuclease-Free Water5µl[γ-32P]ATP (3,000Ci/mmol at 10mCi/ml)1µlT4 Polynucleotide Kinase (5–10u/µl)1µlTotal volume10µl2.To label the probe with biotin, please referred to the EMSA Probe Biotin Labeling Kit (, GS008).3.For cold competition reaction setup, the amount of unlabeled probe should be 50-100 times that of labeled probe.