无花果蛋白酶 来源于无花果树乳胶

F755267-200U

200U

saline suspension,≥1.0 units/mg protein (biuret)

Ficin from fig tree latex has been used:in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionalityfor the digestion of eye tissue sections prior to immunostaining and immunofluorescence应用： Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.消光系数： E1% = 21.0 (280 nm)pI: 9.0 Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges. Extinction Coefficient: E 1% = 21.0 (280 nm) pI: 9.0 Application: Ficin from fig tree latex has been used: in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 K m 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′) 2 片段。

4077