β-葡萄糖醛酸酶 来源于大肠杆菌

G755237-5KU

5KU

aqueous glycerol solution,≥5,000,000 units/g protein, pH 6.8 (biuret)

β-来自大肠杆菌的葡萄糖醛酸酶已用于非小细胞肺癌细胞、U87人胶质母细胞瘤细胞系、A549（人肺腺癌）和KB（人口腔鳞状细胞癌）中葡萄糖醛酸苷前药的酶促裂解和活化。β来自大肠杆菌的-葡萄糖醛酸酶被用于水解尿液和其他液体中的b-葡糖苷酸。 它具有高水解活性，可用于测定尿液是否含有雄甾酮、17-羟基皮质类固醇和雌三醇。采用β-葡萄糖醛酸酶IX-A型确定了人尿中三唑仑主要代谢产物之一α-羟基三唑仑酶解的最佳条件。 在GUS测定中可用作报告基因以监测基因的表达。比较不同酶性能的新技术文章了解更多 了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响有效水解类固醇葡糖苷酸。用于尿液代谢物分析中葡糖苷酸结合物的水解应用： β-来自大肠杆菌的葡萄糖醛酸酶与人葡萄糖醛酸酶相似，并且对应于接近69-71 kDa的分子量，并且具有6.5-7.5的最佳pH值。属于家族-2糖基水解酶，具有活性位点残基谷氨酸394、酪氨酸468和谷氨酸504。 β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504. Application: β-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma). β-Glucuronidase from E. coli is used for the enzymatic hydrolysis of b-glucuronides in urine and other fluids. It has a high rate of hydrolytic activity and may be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine. The optimal conditions for the enzymatic hydrolysis of α-hydroxytriazolam, one of the major metabolites of triazolam in human urine, were determined using β-glucuronidase Type IX-A. It is used as a reporter gene in GUS assays to monitor gene expression. New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine Effective in the hydrolysis of steroid glucuronides. Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

β-葡萄糖醛酸酶催化β-葡糖醛酸残基从糖胺聚糖（GAG）的非还原末端水解。 β-葡萄糖醛酸酶是葡萄糖醛酸苷前药的基因介导酶前药治疗的潜在候选酶。 使用基于β-葡萄糖醛酸酶-白蛋白复合物的药物递送可能是治疗实体瘤的有效治疗方法。β-葡萄糖醛酸酶（β-GIc）是一种可催化复合碳水化合物分解的外切糖苷酶。在人体中，它可将结合胆红素转化为非结合的形式，使胆红素利于重吸收。