葡萄糖氧化酶 (GOD)

glucose oxidase | beta-D-glucose:oxygen 1-oxido-reductase | D-glucose-1-oxidase | glucose aerodehydrogenase | glucose oxyhydrase | gox

G774044-50KU

50KU

≥300 U/mg protein

Glucose oxidase (GOD, EC 1.1.3.4) exhibits high specificity and can specifically catalyze the oxidation reaction of β-D-glucose. Its systematic name is β-D-glucose:oxygen 1-oxidoreductase. In the absence of catalase, the core of the reaction is that "β-D-glucose is oxidized by O₂ to gluconolactone, with the simultaneous production of H₂O₂". Reaction Properties Source: spergillus niger Isoelectric point: 5.2 Michaelis constant: 4.9×10^-2 M (D-Glucose) Optimum pH: 4.5~6.5 Fig. 1 Optimum temperature: 40℃~60℃ Fig. 3 pH Stability: 5.0~7.5 (25℃, 18hr Fig. 2 Thermal stability:

≥300 U/mg protein; Unit definition: One unit will oxidize one micromole of glucose per min at pH 5.7 at 37℃.

重组表达

approx 100 kDa (SDS-PAGE)

黑曲霉(Aspergillus niger)