己糖激酶 来源于酵母(冻干)

己糖激酶

ATP:D-Hexose-6-phosphotransferase | Hexokinase from Saccharomyces cerevisiae | Hexokinase

H128554-2.5KU

2.5KU

EnzymoPure™

≥150 units/mg protein

Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP.Catalyzes the phosphorylation of D-hexose sμgars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP. Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively. Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP. Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP. Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively.

利用 ATP 作为磷酸源，在 C6 位催化 D-己糖的磷酸化。磷酸化速率随不同的己糖而变化（pH 7.5，30 °C）：D-果糖 KM：0.33 mM；D-葡萄糖 KM：0.12 mM；D-甘露糖 KM：0.05 mM。酵母己糖激酶存在两种相似的异构体，即 PI 和 PII（A 和 B），等电点分别为 5.25 和 4。分子量：~ 54 kDa（单体）；~ 110 kDa（二聚体）；最佳 pH 值：7.5 至 9.0；消光系数：E1% = 8.85（PI）和 9.47（PII），波长 280 纳米。活化剂：Hexokinase 的活性需要 Mg2+ 离子（KM = 2.6 mM）。儿茶酚胺和相关化合物可激活己糖激酶。 抑制剂：山梨糖-1-磷酸、多磷酸盐、6-脱氧-6-氟葡萄糖、2-C-羟甲基葡萄糖、木糖、来苏糖和硫醇活性化合物（Hg2+ 和 4-氯巯基苯甲酸酯）。