Application： 4-Indolyl acetate has been employed as standard in a protocol for the optimization of gradient reversed phase chromatographic peak capacity for separation of small molecules：Reactant for preparation of: 1) Inhibitors of HIV-1 attachment; 2)Prostanoid EP3 receptor antagonist, as a novel antiplatelet agent; 3) Indolyl-nitroalkanes via N-bromosuccinimide catalyzed synthesis; 4) Psilocybin as a medicinal agent;