中性蛋白酶 NB 来源于溶组织梭菌

N279017-80U

80U

EnzymoPure™

高活性级,≥ 4.00 U/mg

Description Neutral Protease NB is chromatographically purified and contains an increased proteolytic activity. It is largely free from collagenolytic activity (PZ U) and the endotoxin level is very low. Application Neutral Protease NB is, mostly in combination with Collagenase NB 1 or another Collagenase NB product, suitable for cell isolation from various tissue types. Specifications Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum. Neutral protease activity (DMC): ≥ 4.00 U/mg. Largely free of collagenolytic activity. Endotoxin: ≤ 50 EU**/mg (Ph. Eur.) Mr ca. 34 000 • CAS [9001-92-7] References and Definitions Unit definition: *Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). **Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93. Description Neutral Protease NB is chromatographically purified and contains an increased proteolytic activity. It is largely free from collagenolytic activity (PZ U) and the endotoxin level is very low. Application Neutral Protease NB is, mostly in combination with Collagenase NB 1 or another Collagenase NB product, suitable for cell isolation from various tissue types. Specifications Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum. Neutral protease activity (DMC): ≥ 4.00 U/mg. Largely free of collagenolytic activity. Endotoxin: ≤ 50 EU**/mg (Ph. Eur.) Mr ca. 34 000 • CAS [9001-92-7] References and Definitions Unit definition: *Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). **Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.