Nanaomycin A is the first selective DNMT3B inhibitor with an IC 50 of 500 nM. Nanaomycin A, a quinone antibiotics , reactivates silenced tumor suppressor genes in human cancer cells Nanaomycin A inhibits in vitro growth of the human malaria parasite Plasmodium falciparum with an IC 80 value of 33.1 nM . In Vitro Nanaomycin A (10-10000 nM; 72 hours) has a distinct cytotoxic effect in all three cell lines. Nanaomycin A (0.5, 5 μM; 72 hours) reveals induction of RASSF1A protein expression in A549 cells. Nanaomycin A (5 μM; 72 hours) has an 18-fold relative induction. Nanaomycin A not affects the enzymatic activity of DNMT1. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity AssayCell Line: HCT116 (colon), A549 (lung), and HL60 (bone marrow) human tumor cell lines Concentration: 10, 100, 1000, 10000 nM Incubation Time: 72 hours Result: Had a distinct cytotoxic effect in all three cell lines. Western Blot AnalysisCell Line: A549 cells Concentration: 0.5, 5 μM Incubation Time: 72 hours Result: Revealed induction of RASSF1A protein expression. RT-PCRCell Line: A549 cells Concentration: 5 μM Incubation Time: 72 hours Result: Had an 18-fold relative induction. Form:Solid IC50& Target:DNMT3B 500 nM (IC 50 ) Plasmodium