辣根过氧化物酶(HRP)

过氧化酶

HRP | MFCD10565970 | MGCD0103 - Mocetinostat | NCGC00263182-11 | UNII-A6GWB8T96J | DTXSID80222945 | HMS3426I07 | STK387500 | SCHEMBL157027 | AKOS015916602 | MG 0103 | BRD-K16485616-001-04-8 | FT-0627651 | HRNLUBSXIHFDHP-UHFFFAOYSA-N | MOCETINOSTAT [INN] |

P105525-100mg

100mg

EnzymoPure™

＞200 U/mg, RZ 2-4

辣根过氧化物酶（HRP）广泛应用于液体临床化学试剂、干粉试剂和试纸条中。它也常用作免疫测定（如ELISA）、印迹和组织化学中的标记。一旦与抗体、抗原或链霉亲和素结合，其快速周转率在短孵育时间内具有高灵敏度。过氧化物酶偶联物可能比其他酶偶联物提供更高的背景，因此可能需要额外的阻断步骤，例如引入APO过氧化物酶。它可以与多种产生沉淀或可溶性发色剂的底物一起使用，或者与化学发光和荧光底物一起使用以增强灵敏度。 Horseradish Peroxidase (HRP) is used in a wide range of liquid clinical chemistry reagents, dry powder reagents and test strips. It is also commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its rapid turnover rate gives high sensitivity over short incubation times. Peroxidase conjugates may give higher backgrounds than other enzyme conjugates, hence extra blocking steps, e.g. the introduction of APO Peroxidase, may be necessary. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity.

辣根过氧化物酶与底物孵育时，产生一种着色、荧光或发光标记的分子的衍生物，以便可以定量。辣根过氧化物酶已被证明抑制cydAB突变，使其突变水平稍微降低。已知其抑制剂是叠氮化钠，氰化物，L-胱氨酸，重铬酸盐，亚乙基硫脲，羟基胺，硫化物，钒酸，对氨基苯甲酸，和Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, 和 Pb2+离子。

>200 U/mg

NA.54