蛋白酶 来源于链霉菌 属

P755282-500unit

500unit

Type XXI,≥15 units/mg solid

链霉蛋白酶E可用于降解柞蚕丝素蛋白膜。 至少包含三种蛋白水解活性的混合物，其中包含细胞外丝氨酸蛋白酶。通常，丝氨酸蛋白酶显示出广泛的底物特异性，而这被视为通过分子中由一个Asp、一个His和Ser残基组成的活性位点所介导的。该酶能够选择水解谷氨酸或天冬氨酸的羧基侧的肽键。蛋白酶通常用于核酸的分离过程，通过补充0.2％十二烷基硫酸钠和10 mM EDTA并孵育0.5-3.0小时。该酶可用于不溶性蛋白质的蛋白水解和蛋白结构研究。来自 灰色链霉菌 和 链霉菌 的蛋白酶已经在一项研究中用于鉴定残留物71（作为拓扑特异性差异的关键残留物）。 蛋白酶也已用于研究蛋白酶作为链霉素生产中副产物的产生。 通过在80 °C以上加热15-20分钟以完全灭活。 Pronase E can be used to degrade antheraea pernyi silk fibroin films. A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid. Completely inactivated by heating above 80 °C for 15-20 minutes. Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.

碱性蛋白酶在pH 11.0下的活性约为蛋白酶在pH 7.5和37℃的常规测定条件下的两倍。相比之下，P5147型XIV蛋白酶在pH 11.0,30℃条件下仅有约25％的活性。