胃蛋白酶−琼脂糖 来源于猪胃粘膜

P755312-10KU

10KU

lyophilized powder,≥30 units/mg dry solid

固定在琼脂糖珠上的胃蛋白酶可用于消化并随后从糖蛋白麦醇溶蛋白中分离出活性肽。 用于产生抗体的F(ab′)2 片段。1胃蛋白酶-琼脂糖已作为固定工具用于研究胃蛋白酶的重折叠能力。它还用于从兔IgG制备F(ab′)2片段。应用： 胃蛋白酶是一种在胃液分泌物中发现的酶。 Pepsin is an enzyme found in gastric secretion. Application: Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin. Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG. Used to produce F(ab′) 2 fragments of antibodies. 1

优先将C末端切割成Phe、Leu和Glu。它不会在Val、Ala或Gly上进行切割。最佳pH2-4。活性成分为4 M尿素和3 M盐酸胍。在60°C时稳定。胃蛋白酶在pH > 6时会不可逆地失活。