磷酸酶底物

P755417-25tabs

25tabs

40 mg tablets

磷酸酶底物已用作血清样品、骨髓基质细胞和人间充质干细胞（hMSC）中碱性磷酸酶测定的底物。磷酸酶是一种发色底物，可用于碱性和酸性磷酸酶 ELISA 试验。它用于比色法检测 CTB 融合蛋白。产生黄色的可溶性最终产物，可以在 405 nm 处用分光光度法进行读数。可用 3 N NaOH 终止 pNPP 反应，并在 405 nm 处读数。该底物已用于确定人成骨细胞样 Saos-2 细胞裂解物中的碱性磷酸酶（ALP）活性。ALP 作为成骨细胞分化的早期标志物，该标志物在基质矿化开始之前表达。用于碱性和酸性磷酸酶ELISA法的发色底物。产生可溶性终产物，黄色，分光光度法在405nm处读取。pNPP反应能被3N NAOH终止，在405nm处读取。应用： 人们根据文献和蛋白质-蛋白质相互作用（PPI）数据库绘制了人磷酸酶-底物网络。根据基因表达数据，可以预测磷酸酶-底物激酶的可能网络。 Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted. Application: Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins . Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm. Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC). The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins. Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

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