SIGMAFAST^™ 对硝基苯基磷酸酯片 | SIGMAFAST ™ 对硝基苯基磷酸酯片

P755479-5T

5T

tablet, To prepare 5 mL

SIGMA FAST p-磷酸硝基苯酯片剂用于： 定量非分泌型碱性磷酸酶，监测成骨样细胞的分化情况（SaoS-2）染色小鼠前成骨细胞 MC3T3-E1，进行碱性磷酸酶测定用作 ExtrAvidin 碱性磷酸酶的酶作用物®，通过酶联免疫吸附法（ELISA）测定血红蛋白结合α-突触核蛋白 用作碱性磷酸酶偶联抗体的酶作用物，进行Abirin毒素的ELISA检测开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP，0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取，或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水，即可制备活性底物溶液。片剂单独包装在铝箔包装中 。 SIGMA FAST p -Nitrophenyl phosphate Tablets has been used: to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2) to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay as a substrate for ExtrAvidin alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA) as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMA FAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.