丙酮酸激酶 (PK)

pyruvate kinase | phosphoenolpyruvate kinase | phosphoenol transphosphorylase | PKM | PKM2

P774071-1KU

1KU

生物活性

≥90%(SDS-PAGE)

Pyruvate kinase (PK, EC 2.7.1.40) is a key enzyme in the glycolytic pathway, and its standard catalytic reaction is as follows: Phosphoenolpyruvate (PEP) + ADP → Pyruvate + ATP. Properties Source: Microorganism Isoelectric point: 5.2 Michaelis constant: 1.1×10^-3 M (ADP); 2.2×10^-3 M (PEP) Optimum pH: 7.5 Fig. 1 Optimum temperature: 65℃ Fig. 3 pH Stability: 5.0~10.0 (37℃, 20hr) Fig. 2 Thermal stability:

≥300 U/mg protein; Unit definition: One unit will convert one micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30℃.

重组表达

68 kDa (SDS-PAGE)

NONH for all modes of transport