Information Unesbulin (PTC596) Unesbulin (PTC596) is a second-generation BMI-1 inhibitor that accelerates BMI-1 degradation. PTC596 downregulates MCL-1 and induces p53-independent mitochondrial apoptosis . IC50 values at 72 hours ranged from 68 to 340 nM in mantle cell lymphoma (MCL) cell lines. Targets BMI-1 In vitro PTC596 induces mitochondrial apoptosis in BMI-1-expressing MCL cells in a p53-independent manner. MCL SP cells were susceptible to PTC596-induced apoptosis. In vivo PTC596 inhibits myeloid leukemia cell growth in vivo while sparing normal hematopoietic cells. Cell Research(from reference) Cell lines：Z-138 and REC-1 cells Concentrations：300 nM Incubation Time：24 h