核糖核酸酶T1 来源于米曲霉(色谱纯化,冻干)

RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase

RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase

R128612-2500KU

2500KU

EnzymoPure™

≥2,500 units/mg

Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies . Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA. Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

来自黑曲霉（Aspergillus oryzae）的核糖核酸酶 T1（RNase T1）是一种内切核糖核酸酶，可水解 G 残基。裂解发生在胍核糖核苷酸的 3'- 磷酸基团和相邻核苷酸的 5'- 羟基之间。最初的产物是 2'：3'环磷酸核苷，然后水解为相应的 3'-核苷磷酸。它与胰腺 RNase 的不同之处在于，它专门攻击鸟嘌呤位点，生成 3′-GMP 和带有 3′-GMP 末端基团的寡核苷酸。