速溶型蛋白上样缓冲液（变性, 还原型, 5×）

生物试剂/缓冲液

Protein Sample Loading Buffer (Denaturing, Reducing, 5×)

S750308-1ml

1ml

BioReagent

5×

-20°C储存

超低温冰袋运输

Store at 2-8℃ short term (3 months). Store at -20℃ long term (12 months).

本品用于蛋白质SDS-PAGE电泳时的蛋白上样操作。其采用新型配方，确保煮样后pH值为中性，避免蛋白降解。从-20℃取出后融化速度快，节省等待时间。采用新型还原剂，在保证强效还原性的同时，避免了β-巯基乙醇或DTT的臭味。 产品特点 1、抑制降解——采用新型配方，确保煮样后pH值为中性，避免蛋白降解，尤其适用于磷酸化蛋白； 2、沉降迅速——样品会快速沉到加样孔底，不易飘起； 3、快速融化——从-20℃取出后融化速度快，不粘稠； 4、无异味——采用新型还原剂，不含β-巯基乙醇或DTT，无令人不快的臭味。 使用说明 1、使用时，在蛋白质样品中加入1/4体积本产品，混匀； 2、将混合物用沸水浴加热5~10min； 3、加热结束后，高速离心5min，上清即可用于电泳分析。如蛋白样品在加入上样缓冲液后，无不溶性残渣，则只需短暂离心，将混合液收集到管底即可。加热后，如果管盖上出现较多冷凝水，建议轻弹离心管将冷凝水和样品混合以后，再进行高速离心步骤，此举有助于降低上样样品浓度不均的现象。 注意事项 1、由于含有还原剂，使用本品上样的凝胶在银染时可能会发黄或出现条纹； 2、由于含有变性剂，本品不适用于非变性胶的电泳； 3、为了您的安全和健康，请穿实验服并戴一次性手套操作； 4、本产品仅限科研使用。 This product is designed for protein sample preparation in SDS-PAGE electrophoresis. Its novel formulation ensures a neutral pH after boiling, preventing protein degradation. It features fast thawing from –20°C and eliminates unpleasant odors by replacing traditional reducing agents (e.g., β-mercaptoethanol or DTT) with an advanced odorless alternative. Key Features 1.Prevents Degradation: Neutral pH after boiling avoids protein degradation, ideal for phosphorylated proteins. 2.Rapid Settlement: Samples sink quickly to the bottom of wells without floating. 3.Fast Thawing: Rapid melting from –20°C without viscosity. 4.Odorless: Contains a novel reducing agent—no β-mercaptoethanol or DTT, no unpleasant smell. Protocol 1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly. 2.Heat the mixture in a boiling water bath for 5–10 minutes. 3.Centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis. 3.1Note: If no insoluble debris is observed after adding loading buffer, brief centrifugation is sufficient to collect the mixture. 3.2If condensation forms on the tube lid after heating, gently flick the tube to mix condensation with the sample before centrifugation to ensure uniform loading concentration. Precautions 1.Gels loaded with this buffer may exhibit yellowing or streaks in silver staining due to the reducing agent. 2.Not suitable for non-denaturing gel electrophoresis as it contains denaturing agents. 3.Wear a lab coat and disposable gloves for safety. 4.For research use only.