OSMI-1 是一种细胞渗透性 O-GlcNAc transferase (OGT) 的抑制剂，对全长人OGT (ncOGT) 的IC50值为2.7 μM。OSMI-1 抑制哺乳动物细胞系中的蛋白 O-连接的 N-乙酰氨基葡萄糖 (O-GlcNAcylation)，而没有定性地改变细胞表面 N-或 O-连接的聚糖。 应用： OSMI-1（OGT的小分子抑制剂）已可用于邻位连接分析、Sox2（SRY（性别决定区Y）-Box 2）双荧光素酶报告基因检测、Sox2 ELISA（酶联免疫吸附测定）以及其他 体内 方法 ，以研究 O-连接的β-N-乙酰氨基葡萄糖（O-GlcNAc）介导的癌症自我更新基因的调控。它还被用于研究由O-GlcNAc转移酶介导的Hippo途径调节。 OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC50 value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycan. Application： OSMI-1 (OGT with a small molecule inhibitor) has been used in proximal ligation assay, Sox2 (SRY (sex determining region Y)-Box 2 ) dual luciferase reporter assay, Sox2 ELISA (enzyme linked immunosorbent assay) and other in vivo approaches, to study the O-Linked β-N-acetylglucosamine (O-GlcNAc) mediated regulation of self-renewal genes in cancer. It has also been used to study the Hippo pathway regulation mediated by O-GlcNAc transferase.