泛素激活酶UBE1（人重组）

U388907-50μl

50μL

EnzymoPure™

0.5 μg/μl in PBS containing 0.01% sodium azide

UBE1负责泛素-蛋白质异肽键形成的第一步。UBE1通过形成高能硫酯键来催化遍在蛋白C端羧基的活化。然后，该活化的遍在蛋白通过E2 / E3偶联级联转移到靶蛋白的赖氨酸上。引发体外反应的关键成分。 This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.