C20 Ceramide (d18:1/20:0) or N-arachidoyl-D-erythro-sphingosine is a synthetic ceramide containing sphingosine with arachidonic acid. Ceramide synthase 2 is responsible for the synthesis of C20 ceramide C20 Ceramide (d18:1/20:0) has been used: • as a standard to quantify ceramide content in muscle tissue samples using high-performance liquid chromatography/tandem mass spectrometry (LC/MS2) • as a standard for the quantitation of sphingolipid metabolites • to study its effects on mitochondrial-to-cytosolic stress response (MCSR) induction C20 Ceramide (d18:1/20:0) or N-arachidoyl-D-erythro-sphingosine is a synthetic ceramide containing sphingosine with arachidonic acid. Ceramide synthase 2 is responsible for the synthesis of C20 ceramide C20 Ceramide (d18:1/20:0) has been used: • as a standard to quantify ceramide content in muscle tissue samples using high-performance liquid chromatography/tandem mass spectrometry (LC/MS2) • as a standard for the quantitation of sphingolipid metabolites • to study its effects on mitochondrial-to-cytosolic stress response (MCSR) induction