组织蛋白酶D,人肝

np001110-10μg

10μg

≥95%(SDS-PAGE)

组织蛋白酶D是一种与组织分解相关的雌激素调节蛋白。组织蛋白酶D水平与淋巴结阴性和淋巴结阳性类型的复发性乳腺癌呈正相关。此外，组织蛋白酶D与阿尔茨海默氏症斑块中淀粉样蛋白的形成有关。由于香烟烟雾会增加组织蛋白酶D的活性，这种酶可能会导致吸烟者的肺组织损伤。 活性：>=300单位/毫克蛋白质。一个单位被定义为消化血红蛋白释放肽的酶的量，这些肽可溶于10%TCA。通过在37°C下每60分钟增加1.0的A280来测量反应。底物：酸变性血红蛋白，pH 1.8（反应混合物中0.2%）。缓冲液：100 mM甲酸盐，pH 3.3。 Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer's plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers. Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3. Product Citations: Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease." Neuromolecular medicine (2013): 1-11 Appelqvist, Hanna, et al. "Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of bid at Phe24, Trp48, and Phe183."Annals of Clinical & Laboratory Science 42.3 (2012): 231-242. References: Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209. Barrett, A.J. 1970. Biochemistry. 117, 601. Tandon, A.K. et al. 1990. N. Engl. J. Med. 322, 297. Cataldo, A.M. et al. 1990. Brain Res. 513, 181. Chang, J.C. et al. Am. Rev. Respir. Dis. 140, 958.

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