Recombinant Human Lipocalin-2/NGAL Protein

重组人Lipocalin-2 / NGAL蛋白 | 重组人脂质运载蛋白2 | 重组人脂质运载蛋白-2/NGAL 蛋白

24p3 | 25 kDa alpha 2 microglobulin related subunit of MMP9 | 25 kDa alpha-2-microglobulin-related subunit of MMP-9 | Alpha 2 microglobulin related protein | HGNC:6526 | HNL | Lcn 2 | Lcn2 | Lipocalin-2 | Migration stimulating factor inhibitor | MSFI | Ne

rp148318-50μg

50μg

无动物源

≥97%(SDS-PAGE)

＞97%(SDS-PAGE)

Purity >97% SDS-PAGE. Actual molecular weight 25.7 kDa Molecular weight informat ~ 25.7 kDa (monomer). Human lipocalin-2 (aa 1-198) is fused at the C-terminus to a polyhistidine tag. Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3 .

重组表达

22.7 kDa

C-His

24.6 kDa, under reducing conditions.

HEK293 Accession #: P80188 | HEK293

<1.0 EU/μg

人(Human)

21-198 aa

QDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDGADDDDKAHHHHHHHHHH

Yes

Yes