Recombinant Human RGM-A Protein

重组人RGM-A蛋白 | 重组人反义导向分子A蛋白

RGM domain family member A | Repulsive Guidance Molecule A | RGM domain family member A | RGM domain family, member A | RGMA | RGM-A | RGMrepulsive guidance molecule A | RGM

rp150956-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.

重组表达

42.4 kDa

C-His

25-37 kDa under reducing conditions.

HEK293 Accession #: Q96B86 | HEK293

<0.1 EU/μg

人(Human)

48-422 aa & 169-422 aa

CKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPGHHHHHH

Yes

Yes