Recombinant Human SDF1 Protein

重组人SDF1蛋白

C-X-C motif chemokine 12 | hIRH | hSDF-1 | Intercrine reduced in hepatomas | IRH | PBSF | Pre-B cell growth-stimulating factor | SDF-1

rp151342-50μg

50μg

无动物源

≥95%(SDS-PAGE)

＞95% SDS-PAGE

Purity >95% SDS-PAGE. Additional sequence information Mature chain SDF1 alph Function Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Post-translational Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.

Fully biologically active determined by the dose dependant migration of THP-1 cells. The optimum chemotaxis of SDF1 occurs at 10-60 ng/mL.

重组表达

9.1kDa

N-His

9.9 kDa, under reducing conditions; 9.9 kDa, under non-reducing conditions.

E. coli Accession #: P48061 | E. coli

<1.0 EU/μg

人(Human)

23-89 aa

PVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

Yes

Yes