Recombinant Mouse FGF-21 Protein

重组小鼠FGF-21蛋白

FGF21 | FGF-21 | fibroblast growth factor 21 | FGF 21 | FGF21_HUMAN | FGFL | PRO10196 | UNQ3115 | Fgf8c

rp153970-5μg

5μg

无动物源

≥96%(SDS-PAGE&HPLC)

＞96%(SDS-PAGE, HPLC)

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression and the activity depends on the presence of KLB. FGF-21, in the presence of β-Klotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Murine FGF-21 shows limited binding to heparin. In addition, Murine FGF-21 respectively shows 81% and 92% a.a. identity to human and rat FGF-21, and it show activity on human and rat cells. Recombinant Murine FGF21 is a 19.9kDa globular protein containing 182 amino acid residues. Purity >96%(SDS-PAGE, HPLC) Additional sequence information A single non-glycosylated polypeptide chain containing 182 amino acids. This product is for the mature full length protein. The signal peptide is not included. Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of >2.0 ×10³IU/mg in the presence of 5 µg/ml of rMuβ-Klotho and 10 μg/ml of heparin.

重组表达

19.9 kDa

No tag

E. coli Accession #: Q9JJN1 | E. coli

<0.1 EU/μg

小鼠(Mouse)

29-210 aa

AYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGAAHRS PESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFREL LLEDGYNVYQSEAHGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEP QDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS

Yes

Yes