Recombinant Mouse Osteopontin/OPN Protein

重组小鼠Osteopontin/OPN蛋白 | 重组小鼠骨桥素蛋白

Secreted Phosphoprotein 1 [BNSP] | BNSP | Bone sialoprotein 1 | Eta-1 | MGC110940 | Nephropontin | OPN | Osteopontin | secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1) | secreted phosphoprotein-1 (osteopontin, bone sialoprotei

rp154450-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 ℃. The ED50 for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.

重组表达

31.5 kDa

C-His

30 kDa and 65 kDa under reducing conditions.

HEK293 Accession #: Q547B5 | HEK293

<0.1 EU/μg

小鼠(Mouse)

17-294 aa (E99G)

LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAGSEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVNHHHHHH

Yes

Yes