Recombinant Human IL-1β Protein

重组人IL-1β蛋白 | 重组人IL-1β蛋白

IL1 beta | IL-1 beta | IL-1 | IL1B | IL-1b | IL1-BETA | IL-1F2 | IL1F2IL-1 beta | interleukin 1, beta | interleukin-1 beta | preinterleukin 1 beta | pro-interleukin-1-beta | catabolin

rp156012-10μg

10μg

无载体

≥95%(SDS-PAGE)

Protein Function: IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in humans. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.

重组表达

17.4 kDa

No tag

15.0 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

E. coli Accession #: P01584 | E. coli

<1.0 EU/μg

人(Human)

117-269 aa

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

No

Yes