Recombinant Human GDNF Protein

重组人 GDNF蛋白 | 重组人胶质细胞源性神经营养因子

Astrocyte-derived trophic factor | ATF | ATF1 | ATF2 | GDNF | glial cell derived neurotrophic factor | glial cell line derived neurotrophic factor | glial cell line-derived neurotrophic factor | HFB1-GDNF | HGDNF | HSCR3

rp156172-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Immobilized Recombinant Human GFR alpha-1/GDNF R alpha-1 Protein (rp146402) at 2.0 μg/mL can bind Recombinant human GDNF protein (rp156172) with the EC50 of 3.4 ng/mL.

重组表达

11.7 kDa

No tag

16.4 kDa, under reducing conditions; 31.5 kDa, under non-reducing conditions.

CHO Accession #: P39905 | CHO

<1.0 EU/μg

人(Human)

109-211 aa

GRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

Yes

Yes